.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.48.5. .TH LAMASSEMBLE "1" "July 2022" "lamassemble 1.4.2" "User Commands" .SH NAME lamassemble \- Merges overlapping "long" DNA reads into a consensus sequences .SH SYNOPSIS .B lamassemble [\fI\,options\/\fR] \fI\,last-train.out sequences.fx > consensus.fa\/\fR .SH DESCRIPTION Merge DNA sequences into a consensus sequence. .SH OPTIONS .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-a\fR, \fB\-\-alignment\fR print an alignment, not a consensus .TP \fB\-c\fR, \fB\-\-consensus\fR just make a consensus, of already\-aligned sequences .TP \fB\-g\fR G, \fB\-\-gap\-max\fR=\fI\,G\/\fR use alignment columns with <= G% gaps (default=50) .TP \fB\-\-end\fR \&... including gaps past the ends of the sequences .TP \fB\-s\fR S, \fB\-\-seq\-min\fR=\fI\,S\/\fR omit consensus flanks with < S sequences (default=1) .TP \fB\-n\fR NAME, \fB\-\-name\fR=\fI\,NAME\/\fR name of the consensus sequence (default=lamassembled) .TP \fB\-o\fR BASE, \fB\-\-out\fR=\fI\,BASE\/\fR just write MAFFT input files, named BASE.xxx .TP \fB\-p\fR P, \fB\-\-prob\fR=\fI\,P\/\fR use pairwise restrictions with error probability <= P (default=0.002) .TP \fB\-d\fR D, \fB\-\-diagonal\-max\fR=\fI\,D\/\fR max change in alignment diagonal between pairwise alignments (default=1000) .TP \fB\-v\fR, \fB\-\-verbose\fR show progress messages .TP \fB\-\-all\fR use all of each sequence, not just aligning part .TP \fB\-\-mafft\fR=\fI\,ARGS\/\fR additional arguments for MAFFT .IP LAST options: .TP \fB\-P\fR P number of parallel threads (default=1) .TP \fB\-W\fR W use minimum positions in length\-W windows (default=19) .TP \fB\-m\fR M max initial matches per query position (default=5) .TP \fB\-z\fR Z max gap length (default=30)